![]() ![]() Inheritance of resistance to foliar infection by Xanthomonas axonopodis pv. Genetic basis of resistance to systemic infection by Xanthomonas axonopodis pv. European Journal of Plant Pathology, 121, 35–42.Įlibox, W., & Umaharan, P. A quantitative screening method for the detection of foliar resistance to Xanthomonas axonopodis pv. A green fluorescent protein-based screening method for identification of resistance in anthurium to systemic infection by Xanthomonas axonopodis pv. European Journal of Plant Pathology, 106, 291–295.Įlibox, W., & Umaharan, P. Survival of the anthurium blight pathogen, Xanthomonas axonopodis pv. Comparative and collaborative studies for the validation of a nested PCR for the detection of Xanthomonas axonopodis pv. Plant Pathology, 62, 475–484.Ĭhabirand, A., Jouen, E., Pruvost, O., Chiroleu, F., Hostachy, B., Bergsma‐Vlami, M., et al. Genomics‐informed design of loop‐mediated isothermal amplification for detection of phytopathogenic Xanthomonas arboricola pv. Applied and Environmental Microbiology, 60, 377–384.īühlmann, A., Pothier, J. ![]() dieffenbachiae with potential use for strain identification and characterization. Isolation of an insertion sequence ( IS1051) from Xanthomonas campestris pv. Plant Disease, 98, 909–915.īerthier, Y., Thierry, D., Lemattre, M., & Guesdon, J. Development of a genomics-based LAMP (Loop-mediated isothermal amplification) assay for detection of Pseudomonas fuscovaginae from rice. R., Stodart, B., Verdier, V., Vera Cruz, C., et al. Evolutionary and experimental assessment of novel markers for detection of Xanthomonas euvesicatoria in plant samples. Applied and Environmental Microbiology, 77, 5619–5628.Īlbuquerque, P., Caridade, C. fuscans with novel markers and using a dot blot platform coupled with automatic data analysis. Identification of Xanthomonas fragariae, Xanthomonas axonopodis pv. In all, this study provided a promising and practical molecular tool for Xad detecting, will facilitate the forecasting and control of anthurium blight disease.Īlbuquerque, P., Caridade, C. In addition, combining with the optimized DNA extraction, Xad-LAMP detections were successfully performed on both latent and disease samples, derived from artificially and naturally infected plants respectively. pure genome DNA or 10 4 CFU/ml cells, 10–100 times more sensitive than conventional PCR. In sensitivity testing, Xad-LAMP allowed detection as low as 1–10 f. ![]() The specificity of Xad-LAMP primers set was widely validated on Xad and nontarget strains. The Xad-LAMP reaction could be finished by incubating at 61–65 ☌ for 1 h, and the amplificons were confirmed through gel electrophoresis, HpaII enzyme analysis, and visually inspected using SYBR Green I/calcein stain and lateral flow dipstick (LFD) assay. In this paper, we presented a novel molecular method to detect Xad in anthurium using loop-mediated isothermal amplification (LAMP) technique ( Xad-LAMP). Detection of infection (latent) in anthurium plants is critical to evaluate disease progress and strengthening management to avoid a serious epidemic in the fields. dieffenbachiae ( Xad) is an important and destructive disease worldwide, and no effective technique has been developed for its control. Anthurium bacterial blight caused by Xanthomonas axonopodis pv. ![]()
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